The NBC comedy show - which was created by Tina Fey and stars Alec Baldwin and Tracy Morgan - was recently recommissioned for another run of 13 episodes, but the network have now confirmed that they will be the last, confirming recent speculation about the show's future.
Despite the news, NBC are hopeful of working with Tina - who loosely based the show on her experiences working on Saturday Night Live - on other projects.
NBC executive Bob Greenblatt said: "I know Tina, Alec and the rest will deliver some of their best work.
"We think the world of Tina Fey, who is a comic genius, and we hope she'll be a part of the NBC family for years to come."
30 Rock will draw to a close with a special hour-long season finale.
The news comes after Alec - who plays television executive Jack Donaghyin in 30 Rock - recently hinted he was set to leave the NBC comedy.
The 54-year-old actor hit out at the network last month after The Today Show sent a news crew to his house following the arrest of a French-Canadian woman on charges of stalking him, so he said he was leaving the network "just in time".
He previously wrote on Twitter: "Outside my apt today, along side the other stalkers from the tabloid press, a crew that identified themselves as being with the Today Show.
"The television crew camped outside my apt said they were with the Today Show. #howthemightyhavefallen. I haven't appeared on the Today Show in many years. But did they have to camp outside my apt?
"A story about stalking sure brings out the stalkers in the media. But, the Today Show? No one from NBC contacted me until the Today Show arrived at my apt. I think I'm leaving NBC just in time (sic)."
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