The Harry Potter writer agreed to take part in the series - which sees celebrity participants research their family history - to discover more about her late mother Anne's French ancestry, and her episode sees her visit Edinburgh and London before travelling to Paris.
Also featuring in the next series of the BBC One show is actor Larry Lamb, who traces a long-lost relative and learns the story behind his mother's adoption.
Other stars who will examine their backgrounds on the programme include Strictly Come Dancing judge Len Goodman, Bee Gees singer Robin Gibb, TV stars Richard Madeley and Alan Carr, actress Emilia Fox, former athlete Lord Sebastian Coe and artist Tracey Emin.
Rowling - whose first name is Joanne - has previously spoken of her regret that her beloved mum, who died from multiple sclerosis aged 45, never got to see any of her Harry Potter books.
She said: "I started writing Harry six months before my mum died. That's obviously a real regret. I never told her I was even writing it.
"She knew I wanted to write. I'm not sure how seriously she took it, but she never knew anything about Harry Potter at all."